Fine detail of neurovascular coupling revealed by spatiotemporal analysis of the hemodynamic response to single whisker stimulation in rat barrel cortex.
J Neurophysiol
; 99(2): 787-98, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-18046008
ABSTRACT
The spatial resolution of hemodynamic-based neuroimaging techniques, including functional magnetic resonance imaging, is limited by the degree to which neurons regulate their blood supply on a fine scale. Here we investigated the spatial detail of neurovascular events with a combination of high spatiotemporal resolution two-dimensional spectroscopic optical imaging, multichannel electrode recordings and cytochrome oxidase histology in the rodent whisker barrel field. After mechanical stimulation of a single whisker, we found two spatially distinct cortical hemodynamic responses a transient response in the "upstream" branches of surface arteries and a later highly localized increase in blood volume centered on the activated cortical column. Although the spatial representation of this localized response exceeded that of a single "barrel," the spread of hemodynamic activity accurately reflected the neural response in neighboring columns rather than being due to a passive "overspill." These data confirm hemodynamics are capable of providing accurate "single-condition" maps of neural activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Córtex Somatossensorial
/
Mapeamento Encefálico
/
Vibrissas
/
Potenciais Somatossensoriais Evocados
/
Hemodinâmica
Limite:
Animals
Idioma:
En
Revista:
J Neurophysiol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Reino Unido