Improvements in the chain-termination method of DNA sequencing through the use of 7-deaza-2'-deoxyadenosine.
DNA Seq
; 1(4): 233-9, 1991.
Article
em En
| MEDLINE
| ID: mdl-1806040
ABSTRACT
Significant improvements in the quality of DNA sequencing data have been shown when deoxyadenosine triphosphate (dATP) is replaced by 7-deaza-2'-deoxyadenosine triphosphate (c7dATP). The use of c7dATP in conjunction with 7-deaza-2'-deoxyguanosine triphosphate (c7dGTP) further decreases anomalies in electrophoretic mobility which are caused by compressions involving G and/or A residues. This effect is observed for both isotope-based and fluorescence-based sequencing approaches. Replacing dATP with c7dATP also results in a higher degree of uniformity in the frequency of chain termination reactions, when such terminations involve the incorporation of fluorescence-labeled dideoxynucleotides by T7 polymerase. These improvements in the gel-resolution and distribution of chain-terminated DNA products result in higher accuracy in both manual and automated base assignment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sequência de Bases
/
Tubercidina
Idioma:
En
Revista:
DNA Seq
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
1991
Tipo de documento:
Article