Vaccination with F1-V fusion protein protects black-footed ferrets (Mustela nigripes) against plague upon oral challenge with Yersinia pestis.
J Wildl Dis
; 44(1): 1-7, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-18263816
ABSTRACT
Previous studies have established that vaccination of black-footed ferrets (Mustela nigripes) with F1-V fusion protein by subcutaneous (SC) injection protects the animals against plague upon injection of the bacterium Yersinia pestis. This study demonstrates that the F1-V antigen can also protect ferrets against plague contracted via ingestion of a Y. pestis-infected mouse, a probable route for natural infection. Eight black-footed ferret kits were vaccinated with F1-V protein by SC injection at approximately 60 days-of-age. A booster vaccination was administered 3 mo later via SC injection. Four additional ferret kits received placebos. The animals were challenged 6 wk after the boost by feeding each one a Y. pestis-infected mouse. All eight vaccinates survived challenge, while the four controls succumbed to plague within 3 days after exposure. To determine the duration of antibody postvaccination, 18 additional black-footed ferret kits were vaccinated and boosted with F1-V by SC injection at 60 and 120 days-of-age. High titers to both F1 and V (mean reciprocal titers of 18,552 and 99,862, respectively) were found in all vaccinates up to 2 yr postvaccination, whereas seven control animals remained antibody negative throughout the same time period.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peste
/
Yersinia pestis
/
Proteínas Recombinantes de Fusão
/
Vacina contra a Peste
/
Furões
/
Anticorpos Antibacterianos
Limite:
Animals
Idioma:
En
Revista:
J Wildl Dis
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos