Possible role of Borrelia burgdorferi sensu lato infection in lichen sclerosus.
Arch Dermatol
; 144(5): 591-8, 2008 May.
Article
em En
| MEDLINE
| ID: mdl-18490585
ABSTRACT
OBJECTIVE:
To assess the evidence for Borrelia burgdorferi sensu lato infection in patients with lichen sclerosus by focus-floating microscopy.SETTING:
Dermatology department of a university hospital.DESIGN:
Tissue sections were stained with a polyclonal B burgdorferi antibody using standard histological equipment and then scanned simultaneously in 2 planes horizontally in a serpentine-like pattern and vertically by focusing through the thickness of the section, ie, focus-floating microscopy. Part of the material was also investigated by Borrelia-specific polymerase chain reaction. PATIENTS The study population comprised 61 cases of lichen sclerosus and 118 controls (60 negative controls and 68 positive controls). MAIN OUTCOMEMEASURE:
The presence of B burgdorferi sensu lato within tissue specimens.RESULTS:
Using focus-floating microscopy, we detected Borrelia species in 38 of 60 cases (63%) of lichen sclerosus and in 61 of 68 (90%) of positive controls of classic borreliosis, but Borrelia species were absent in all negative controls. Borrelia species were detected significantly more often in early inflammatory-rich (31 of 39 [80%]) than in late inflammatory-poor (7 of 21 [33.3%]) cases (P = .001). Polymerase chain reaction findings were positive in 25 of 68 positive controls (37%) and negative in all 11 cases of lichen sclerosus and all 15 negative controls.CONCLUSIONS:
Focus-floating microscopy is a reliable method to detect Borrelia species in tissue sections. The frequent detection of this microorganism, especially in early lichen sclerosus, points to a specific involvement of B burgdorferi or other similar strains in the development or as a trigger of this disease.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doença de Lyme
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Grupo Borrelia Burgdorferi
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Líquen Escleroso e Atrófico
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Child, preschool
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Arch Dermatol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Áustria