Localization of human coagulation factor VIII (hFVIII) in transgenic rabbit by FISH-TSA: identification of transgene copy number and transmission to the next generation.
Folia Biol (Praha)
; 54(4): 121-4, 2008.
Article
em En
| MEDLINE
| ID: mdl-18808737
ABSTRACT
For chromosomal localization of the hFVIII human transgene in F2 and F3 generation of transgenic rabbits, FISH-TSA was applied. A short cDNA probe (1250 bp) targeted chromosomes 3, 7, 8, 9 and 18 of an F2 male (animal 1-3-8). Two transgenic offspring (F3) revealed signal positions in chromosome 3 and chromosomes 3 and 7, respectively. Sequencing and structure analysis of the rabbit orthologous gene revealed high similarity to its human counterpart. Part of the sequenced cDNA (1310 bp) served as a probe for FISH-TSA analysis. The rabbit gene was localized in the q arm terminus of the X chromosome. This result is in agreement with reciprocal chromosome painting between the rabbit and the human. The presented FISH-TSA method provides strong signals without any interspecies reactivity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator VIII
/
Animais Geneticamente Modificados
/
Mapeamento Cromossômico
/
Hibridização in Situ Fluorescente
/
Dosagem de Genes
/
Técnicas de Amplificação de Ácido Nucleico
/
Cromossomos de Mamíferos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Folia Biol (Praha)
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
República Tcheca