Brucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression.
Appl Environ Microbiol
; 74(22): 7051-5, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-18836016
ABSTRACT
To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Brucella abortus
/
Brucelose
/
Vacina contra Brucelose
/
Leucina
Limite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos