Amperometric phenol biosensor based on horseradish peroxidase entrapped PVF and PPy composite film coated GC electrode.
Appl Biochem Biotechnol
; 160(3): 856-67, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-19224403
ABSTRACT
Polyvinylferrocene (PVF) was used as a mediator for the fabrication of a horseradish peroxidase (HRP)-modified electrode to detect phenol derivatives via a composite polymeric matrix of conducting polypyrrole (PPy). Through an electropolymerization process, enzyme HRP was entrapped with PPy in a three-electrode system onto a glassy carbon electrode previously covered with PVF, resulting in a composite polymeric matrix. Steady-state amperometric measurements were performed at -200 mV vs. Ag/AgCl in aqueous phosphate buffer containing NaCl 0.1 M (pH 6.8) in the presence of hydrogen peroxide. The response of the HRP-modified PVF electrode was investigated for various phenol derivatives, which were 4-chlorophenol, phenol, catechol, hydroquinone, 2-aminophenol, pyrogallol, m-cresol, and 4-methoxyphenol. Analytical parameters for the fabricated PVF electrode were obtained from the calibration curves. The highest sensitivity was obtained from the calibration of 4-chlorophenol as 29.91 nA/microM. The lowest detection limit was found to be 0.22 microM (S/N = 3) for catechol, and the highest detection limit was found to be 0.79 microM (S/N = 3) for 4-methoxyphenol among the tested derivatives. The biosensor can reach 95% of steady-state current in about 5 min. The electrode is stable for 2 months at 4 degrees Celsius.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
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Polivinil
/
Pirróis
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Carbono
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Compostos Ferrosos
/
Técnicas Biossensoriais
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Fenol
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Vidro
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Peroxidase do Rábano Silvestre
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Turquia