Detecting a deletion in the coding region of the bovine bone morphogenetic protein 15 gene (BMP15).
J Appl Genet
; 50(2): 145-8, 2009.
Article
em En
| MEDLINE
| ID: mdl-19433911
ABSTRACT
The aim of this study was to detect polymorphism in the bovine bone morphogenetic protein 15 (BMP15) gene. On the basis of PCR-SSCP and DNA sequencing, a 4-bp deletion was identified in the coding region of the gene. Sequence analysis revealed that the deletion altered the reading frame and introduced a stop codon at position 264. Eight breeds (Luxi, Qinchuan, Nanyang, Jinnan, Bohai Black, Menggolian, Holstein, and Simmental) were genotyped by PCR-SSCP. No cows homozygous for this mutation were observed in these breeds. Heterozygous cows were detected in Luxi, Qinchuan, Nanyang, Jinnan and Bohai Black cattle. Fecundity was not increased in heterozygous individuals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fases de Leitura Aberta
/
Deleção de Sequência
/
Proteína Morfogenética Óssea 15
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Appl Genet
Assunto da revista:
GENETICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China