Lymphoproliferative diseases of fowl: JM-V leukemic lymphoblasts in cell culture.
J Natl Cancer Inst
; 59(1): 267-71, 1977 Jul.
Article
em En
| MEDLINE
| ID: mdl-195070
ABSTRACT
JM-V leukemic lymphoblasts were established in cell culture. The cultured cells (JM-VLC cells) were transplantable in young chicks and produced a disease indistinguishable from JM-V lymphoblastic leukemia as initiated by whole-blood inoculation. JM-VLC cells maintained a normal female karyotype through 13 passages in Rhode Island Red cockerels. With the use of JM-V antisera and antisera from birds with naturally occurring Marek's disease (MD), specific antigens were detected on the surfaces of living cells. Intracellular antigens were detected with anti-MD virus sera after cultivation for at least 1 day at 37 degrees C. In spite of the expression of MD antigens, the presence of herpesvirus particles associated with the cultured cells, and the occurrence of foci of multinucleated cells in kidney cultures from chicks inoculated with cellfree preparations of JM-VLC cells, the pathologic potential of the cultured cells was that of JM-V leukemia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Experimental
/
Células Cultivadas
/
Galinhas
Limite:
Animals
Idioma:
En
Revista:
J Natl Cancer Inst
Ano de publicação:
1977
Tipo de documento:
Article