Rab14 from Bombyx mori (Lepidoptera: Bombycidae) shows ATPase activity.
Biol Lett
; 6(3): 379-81, 2010 Jun 23.
Article
em En
| MEDLINE
| ID: mdl-20071392
ABSTRACT
Rab GTPases are essential for vesicular transport, whereas adenosine triphosphate (ATP) is the most important and versatile of the activated carriers in the cell. But there are little reports to clarify the connection between ATP and Rab GTPases. A cDNA clone (Rab14) from Bombyx mori was expressed in Escherichia coli as a glutathione S-transferase fusion protein and purified. The protein bound to [(3)H]-GDP and [(35)S]-GTPgammaS. Binding of [(35)S]-GTPgammaS was inhibited by guanosine diphosphate (GDP), guanosine triphosphate (GTP) and ATP. Rab14 showed GTP- and ATP-hydrolysis activity. The Km value of Rab14 for ATP was lower than that for GTP. Human Rab14 also showed an ATPase activity. Furthermore, bound [(3)H]-GDP was exchanged efficiently with GTP and ATP. These results suggest that Rab14 is an ATPase as well as GTPase and gives Rab14 an exciting integrative function between cell metabolic status and membrane trafficking.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bombyx
/
Adenosina Trifosfatases
/
Proteínas rab de Ligação ao GTP
Limite:
Animals
Idioma:
En
Revista:
Biol Lett
Assunto da revista:
BIOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão