[Molecular characterization of full-length genome of Japanese encephalitis virus isolated in Liaoning Province in 2008].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(4): 248-50, 2009 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-20108764
ABSTRACT
OBJECTIVE:
To sequence and analyze the whole genome of Japanese encephalitis virus (JEV) isolated from mosquitoes in Liaoning province in 2008.METHODS:
Using RT-PCR to amplify fragments with genome sequencing primer. The full-length genome was obtained by sequencing and splicing. The differentiation analysis for nucleotides, deduced amino acid sequence and phylogenetic tree was performed by the software of Clustal X (1.83), ATGC (V4), DNAStar, GENEDOC (3.2) and Mega (4.0).RESULTS:
The whole genome of strain LN0828 possesses 10 965 nucleotides. An open reading frame from 97 to 10 392 including 10 296 nucleotides is capable of coding for a 3432 amino acid polyprotein. Comparison of strain LN0828 genomic sequence with those of 32 JEV isolates in GenBank showed that nucleotide sequence divergence ranges from 1.6% to 16.4%, which resulted in amino acid sequence divergence from 0.3% to 5.1%. In comparison with live attenuated vaccine stain SA14-14-2 in open reading frame, strain LN0828 has a total of 1186 nucleotide substitutions, 86 amino acid divergences. Based on phylogenetic analysis, the strain LN0828 belongs to the genotype I JEV.CONCLUSION:
The whole genome of strain LN0828 is close to those of isolates from Liaoning in 2002 and 2007, which were grouped into genotype I JEV.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Viral
/
Encefalite Japonesa
/
Vírus da Encefalite Japonesa (Espécie)
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Assunto da revista:
VIROLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China