Human cytomegalovirus infection among patients living with AIDS in a tertiary level hospital in India.
J Int Assoc Physicians AIDS Care (Chic)
; 9(2): 94-7, 2010.
Article
em En
| MEDLINE
| ID: mdl-20484735
ABSTRACT
OBJECTIVE:
Detecting the prevalence of human cytomegalovirus (CMV) coinfection in HIV-infected individuals attending a tertiary care hospital in Delhi by using 3 laboratory tests namely pp65 antigenemia assay, CMV DNA polymerase chain reaction (PCR), and immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) for anti-CMV antibody.DESIGN:
HIV seropositive patients with CD4 count < or = 100 cells/mm(3) were included as cases and HIV-negative healthy individuals as controls. Blood samples were collected from all participants and 3 tests were performed.METHODS:
pp65 antigenemia assay and IgM ELISA were performed by commercially available kits; PCR was performed by an in-house nested PCR method.RESULTS:
In all, 7.1%, 5.7%, and 2.9% were positive for pp65 antigenemia, CMV DNA, and IgM antibody against CMV, respectively. On the basis of pp65 and/or PCR positivity, it can be said that active CMV coinfection was present in 8.57% of the cases.CONCLUSION:
In the post-antiretroviral therapy (ART) era, CMV coinfection in HIV-infected patients has significantly declined.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por HIV
/
Infecções Oportunistas Relacionadas com a AIDS
/
Infecções por Citomegalovirus
/
Citomegalovirus
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Int Assoc Physicians AIDS Care (Chic)
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Índia