Electrically sensing protease activity with nanopores.
J Phys Condens Matter
; 22(45): 454103, 2010 Nov 17.
Article
em En
| MEDLINE
| ID: mdl-21339591
ABSTRACT
The enzymatic activity of a protease was electrically detected using nanopore recordings. A peptide substrate was tethered to microscale beads, and cleavage by the enzyme trypsin released a soluble fragment that was electrophoretically driven through the α-hemolysin protein pore, leading to detectable blockades in the ionic current. Owing to its simplicity, this approach to sense enzymatic activity may be applied to other proteases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Hidrolases
/
Porosidade
/
Nanoestruturas
/
Eletroquímica
/
Eletroforese
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Ativação Enzimática
Idioma:
En
Revista:
J Phys Condens Matter
Assunto da revista:
BIOFISICA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Reino Unido