In vivo role of different domains and of phosphorylation in the transcription factor Nkx2-1.
BMC Dev Biol
; 11: 9, 2011 Feb 23.
Article
em En
| MEDLINE
| ID: mdl-21345181
ABSTRACT
BACKGROUND:
The transcription factor Nkx2-1 (also known as TTF-1, Titf1 or T/EBP) contains two apparently redundant activation domains and is post-translationally modified by phosphorylation. We have generated mouse mutant strains to assess the roles of the two activation domains and of phosphorylation in mouse development and differentiation.RESULTS:
Mouse strains expressing variants of the transcription factor Nkx2-1 deleted of either activation domain have been constructed. Phenotypic analysis shows for each mutant a distinct set of defects demonstrating that distinct portions of the protein endow diverse developmental functions of Nkx2-1. Furthermore, a mouse strain expressing a Nkx2-1 protein mutated in the phosphorylation sites shows a thyroid gland with deranged follicular organization and gene expression profile demonstrating the functional role of phosphorylation in Nkx2-1.CONCLUSIONS:
The pleiotropic functions of Nkx2-1 are not all due to the protein as a whole since some of them can be assigned to separate domains of the protein or to specific post-translational modifications. These results have implication for the evolutionary role of mutations in transcription factors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hipófise
/
Glândula Tireoide
/
Fatores de Transcrição
/
Proteínas Nucleares
/
Processamento de Proteína Pós-Traducional
Limite:
Animals
Idioma:
En
Revista:
BMC Dev Biol
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Itália