Crystallization and preliminary X-ray analysis of argininosuccinate lyase from Streptococcus mutans.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 67(Pt 6): 682-4, 2011 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-21636911
ABSTRACT
Argininosuccinate lyase (ASL) is an important enzyme in arginine synthesis and the urea cycle, which are highly conserved from bacteria to eukaryotes. The gene encoding Streptococcus mutans ASL (smASL) was amplified and cloned into expression vector pET28a. The recombinant smASL protein was expressed in a soluble form in Escherichia coli strain BL21 (DE3) and purified to homogeneity by two-step column chromatography. Crystals suitable for X-ray analysis were obtained and X-ray diffraction data were collected to a resolution of 2.5â
Å. The crystals belonged to space group R3, with unit-cell parameters a = b = 254.5, c = 78.3â
Å.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Argininossuccinato Liase
/
Streptococcus mutans
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Ano de publicação:
2011
Tipo de documento:
Article