Oligomerization of MDC1 protein is important for proper DNA damage response.
J Biol Chem
; 286(32): 28192-9, 2011 Aug 12.
Article
em En
| MEDLINE
| ID: mdl-21705321
ABSTRACT
Mediator of DNA damage checkpoint 1 (MDC1) plays an important role in the DNA damage response (DDR). MDC1 functions as a mediator protein and binds multiple proteins involved in different aspects of the DDR. However, little is know about the organization of MDC1 complexes. Here we show that ataxia telangiectasia, mutated (ATM) phosphorylates MDC1 at Thr-98 following DNA damage, which promotes its oligomerization. Oligomerization of MDC1 is important for the accumulation of MDC1 complex at the sites of DNA damage. Mutation of Thr-98 (T98A) would abolish its oligomerization and result in a defect in DNA damage checkpoint activation and increased sensitivity to irradiation. Taken together, these results suggest that the oligomerization of MDC1 plays an important role in DDR and help understand the formation of proteins complexes at the sites of DNA damage.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Proteínas Serina-Treonina Quinases
/
Proteínas de Ciclo Celular
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Proteínas Supressoras de Tumor
/
Peptídeos e Proteínas de Sinalização Intracelular
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Proteínas de Ligação a DNA
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Multimerização Proteica
Limite:
Animals
Idioma:
En
Revista:
J Biol Chem
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China