Cyclosporin A inhibits the replication of diverse coronaviruses.
J Gen Virol
; 92(Pt 11): 2542-2548, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-21752960
ABSTRACT
Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Ciclosporina
/
Vírus da Hepatite Murina
/
Coronavirus Humano 229E
/
Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Holanda