A colorimetric method for determination of γ-glutamyl-S-ethenyl-cysteine in narbon vetch (Vicia narbonensis L.) seeds.
Anal Biochem
; 418(2): 180-3, 2011 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-21839063
ABSTRACT
A new colorimetric method based on the bleaching of the iodoplatinate ion has been developed for fast and easy determination of γ-glutamyl-S-ethenyl-cysteine (GEC) in narbon vetch (Vicia narbonensis L.) seeds. The calibration curve showed a good correlation (r(2)=0.9959) between absorbance and GEC amounts from 5.5 to 33 µg (10-59.78 µmol/L). The limits of detection and quantification were 1.16 and 3.55 µmol/L, respectively, and no significant interferences from other sulfur-containing compounds were observed. The method showed excellent repeatability (relative standard deviation [RSD]=0.28%), reproducibility (RSD=4.4%), and accuracy (94%). Determination of GEC in 20 narbon vetch accessions yielded values that were in agreement with those reported previously using capillary electrophoresis and high-performance liquid chromatography methods. The method could be especially valuable for determination of GEC during the process of production of new low-GEC narbon vetch varieties.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sementes
/
Colorimetria
/
Vicia
/
Dipeptídeos
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Espanha