Imaging of vascular smooth muscle cells with soft X-ray spectromicroscopy.
Microsc Microanal
; 17(6): 991-1001, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-22067812
ABSTRACT
Using X-ray microscopy and spectromicroscopy, vascular smooth muscle cells (VSMCs) were imaged, prepared without using additional embedding material or staining, but by applying simple, noncryo fixation techniques. The cells were imaged with a compact source transmission X-ray microscope and a scanning transmission X-ray microscope (STXM). With the STXM, spectromicroscopy was performed at the C K-edge and the Ca L(III,II)-edges. VSMCs were chosen because of their high amount of actin stress fibers, so that the actin cytoskeleton should be visible. Other parts of the cell, such as the nucleus and organelles, were also identified from the micrographs. Both in the spectra and the images, the effects of the different preparation procedures were observable. Furthermore, Ca hotspots were detected and their density is determined.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto de Actina
/
Organelas
/
Núcleo Celular
/
Miócitos de Músculo Liso
/
Microanálise por Sonda Eletrônica
/
Imagem Molecular
/
Músculo Liso Vascular
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Microsc Microanal
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Alemanha