Effects of 2-methoxyestradiol on proliferation, apoptosis and gene expression of cyclin B1 and c-Myc in esophageal carcinoma EC9706 cells.
Cell Biochem Funct
; 30(2): 158-65, 2012 Mar.
Article
em En
| MEDLINE
| ID: mdl-22095881
ABSTRACT
2-Methoxyestradiol (2-ME) is an endogenous metabolite of 17ß-estradiol. In this study, we determined the antitumour activities of 2-ME on the well-differentiated EC9706 esophageal carcinoma cells in vitro. 2-ME had a strong antiproliferative effect on EC9706 cells and caused an increase in the population of apoptotic cells, detected by flow cytometry. A significant number of cells were blocked in the G(2)/M phase of the cell cycle. 2-ME-treated cells demonstrated an increase in cyclin B1 and c-Myc protein levels, as well as an increase in the percentage of G(2)/M phase. Their up-regulation may be involved in 2-ME-induced apoptosis and G(2)/M cell cycle arrest of the EC9706 cells, and it precedes the onset of apoptosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Esofágicas
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Carcinoma
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Regulação Neoplásica da Expressão Gênica
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Proteínas Proto-Oncogênicas c-myc
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Apoptose
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Proliferação de Células
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Estradiol
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Ciclina B1
Limite:
Humans
Idioma:
En
Revista:
Cell Biochem Funct
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China