Glucose sensing by time-resolved fluorescence of sol-gel immobilized glucose oxidase.
Sensors (Basel)
; 11(4): 3483-97, 2011.
Article
em En
| MEDLINE
| ID: mdl-22163807
ABSTRACT
A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0.17 mM) glucose determination with a detection range from 0.4 mM to 5 mM.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Enzimas Imobilizadas
/
Glucose
/
Glucose Oxidase
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Itália