Campylobacter jejuni infection and virulence-associated genes in children with moderate to severe diarrhoea admitted to emergency rooms in northeastern Brazil.
J Med Microbiol
; 61(Pt 4): 507-513, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22174372
ABSTRACT
Campylobacter is an important cause of foodborne gastroenteritis. We determined the occurrence of Campylobacter jejuni and Campylobacter coli, using culture-based methods and PCRs targeting virulence-associated genes (VAGs) among children aged ≤14 years who were treated for diarrhoea at emergency rooms in northeastern Brazil. Genomic DNA was extracted directly from stool samples collected from 366 children. A questionnaire was also applied to qualify the clinical conditions presented by each child at the time of admission. C. jejuni and C. coli were detected in 16.4â% (60/366) and 1.4â% (5/366) of the diarrhoeal samples, respectively, by PCR, a much higher proportion than that detected by conventional methods. C. jejuni VAGs were detected in the following proportions of hipO-positive samples ciaB, 95â% (57/60); dnaJ, 86.7â% (52/60); racR, 98.3â% (59/60); flaA, 80â% (48/60); pldA, 45â% (27/60); cdtABC, 95â% (57/60); and pVir 0â% (0/60). Particular symptoms, such as blood in faeces, vomiting, fever, and/or abdominal pain, were not associated with detection of C. jejuni nor were they associated with any particular VAG or combination of VAGs (P>0.05). C. jejuni and its VAGs were detected in a substantial proportion of the children admitted. Further efforts shall be directed towards elucidating whether these genetic factors or their expressed proteins play a role in Campylobacter pathogenesis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Campylobacter
/
Campylobacter jejuni
/
Diarreia
/
Serviço Hospitalar de Emergência
Tipo de estudo:
Risk_factors_studies
Limite:
Adolescent
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Med Microbiol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil