Persistence of viral RNA in chikungunya virus-infected Aedes aegypti (Diptera: Culicidae) mosquitoes after prolonged storage at 28°C.
Am J Trop Med Hyg
; 86(1): 178-80, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22232470
ABSTRACT
Experiments were conducted to determine the persistence of chikungunya viral (CHIKV) RNA in experimentally infected Aedes aegypti mosquitoes stored for prolonged periods at 28°C. Intra-thoracically inoculated mosquitoes with confirmed positivity were killed by quick freezing at -80°C, applied to sticky tape, and stored at 28°C with 80 ± 5% relative humidity (RH). At weekly intervals, five mosquitoes were removed from the tape randomly and assayed individually for detection of viral RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). CHIKV RNA was detected up to 12 weeks in dry mosquitoes by RT-PCR. Virus could not be isolated either in cell culture or in the suckling Swiss-albino mouse system at any stage. This study demonstrated the persistence of CHIKV viral RNA up to 12 weeks when stored at 28°C with RH 80 ± 5%. This finding will have significance in CHIKV surveillance programs in mosquito populations or field-based studies in countries where maintenance of a cold chain is a concern.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Manejo de Espécimes
/
RNA Viral
/
Vírus Chikungunya
/
Aedes
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Índia