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Proteomic analysis of α4ß1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment.
Byron, Adam; Humphries, Jonathan D; Craig, Sue E; Knight, David; Humphries, Martin J.
Afiliação
  • Byron A; Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK.
Proteomics ; 12(13): 2107-14, 2012 Jul.
Article em En | MEDLINE | ID: mdl-22623428
ABSTRACT
Integrin adhesion receptors mediate cell-cell and cell-extracellular matrix interactions, which control cell morphology and migration, differentiation, and tissue integrity. Integrins recruit multimolecular adhesion complexes to their cytoplasmic domains, which provide structural and mechanosensitive signaling connections between the extracellular and intracellular milieux. The different functions of specific integrin heterodimers, such as α4ß1 and α5ß1, have been attributed to distinct signal transduction mechanisms that are initiated by selective recruitment of adhesion complex components to integrin cytoplasmic tails. Here, we report the isolation of ligand-induced adhesion complexes associated with wild-type α4ß1 integrin, an activated α4ß1 variant in the absence of the α cytoplasmic domain (X4C0), and a chimeric α4ß1 variant with α5 leg and cytoplasmic domains (α4Pα5L), and the cataloguing of their proteomes by MS. Using hierarchical clustering and interaction network analyses, we detail the differential recruitment of proteins and highlight enrichment patterns of proteins to distinct adhesion complexes. We identify previously unreported components of integrin adhesion complexes and observe receptor-specific enrichment of molecules with previously reported links to cell migration and cell signaling processes. Furthermore, we demonstrate colocalization of MYO18A with active integrin in migrating cells. These datasets provide a resource for future studies of integrin receptor-specific signaling events.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Subunidades Proteicas / Integrina alfa4beta1 / Proteômica / Mapas de Interação de Proteínas Limite: Humans Idioma: En Revista: Proteomics Assunto da revista: BIOQUIMICA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Subunidades Proteicas / Integrina alfa4beta1 / Proteômica / Mapas de Interação de Proteínas Limite: Humans Idioma: En Revista: Proteomics Assunto da revista: BIOQUIMICA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Reino Unido