Autophagy is decreased in mesenteric fat tissue but not in intestinal mucosae of patients with Crohn's disease.
Cell Tissue Res
; 350(3): 549-52, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-22948252
ABSTRACT
Crohn's disease (CD) is a chronic intestinal disease with a multifactorial etiology. Recently, a role for mesenteric fat has been proposed in CD pathophysiology, since fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and the hypertrophied mesenteric tissue in CD. To evaluate autophagy-related proteins in intestinal mucosae and mesenteric fat of patients with CD and controls, patients with ileocecal CD (CD Group) and with non-inflammatory disease (FC Group) selected for surgery were studied. Expression of LC3-II was determined by immunoblotting of protein extracts. In addition, beclin-1, LC3 and Atg16-L1 RNA levels were measured using RT-PCR. The expression of LC3-II was significantly lower in the mesenteric tissue and higher in intestinal mucosae of CD when compared to controls. However, mRNA expression of autophagy-related proteins was similar when comparing the mesenteric fat groups. These findings suggest a defect in autophagy activation in the mesenteric fat tissue of CD individuals, which could be involved in the maintenance of the inflammatory process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Doença de Crohn
/
Tecido Adiposo
/
Mucosa Intestinal
Limite:
Humans
Idioma:
En
Revista:
Cell Tissue Res
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil