The HER2-binding affibody molecule (Z(HER2â¶342))2 increases radiosensitivity in SKBR-3 cells.
PLoS One
; 7(11): e49579, 2012.
Article
em En
| MEDLINE
| ID: mdl-23166716
ABSTRACT
We have previously shown that the HER2-specific affibody molecule (Z(HER2â¶342))2 inhibits proliferation of SKBR-3 cells. Here, we continue to investigate its biological effects in vitro by studying receptor dimerization and clonogenic survival following irradiation. We found that (Z(HER2â¶342))2 sensitizes the HER2-overexpressing cell line SKBR-3 to ionizing radiation. The survival after exposure to (Z(HER2â¶342))2 and 8 Gy (S(8Gy) 0.006) was decreased by a factor four compared to the untreated (S(8Gy) 0.023). The low HER2-expressing cell line MCF-7 was more radiosensitive than SKBR-3 but did not respond to (Z(HER2â¶342))2. Treatment by (Z(HER2â¶342))2 strongly increased the levels of dimerized and phosphorylated HER2 even after 5 minutes of stimulation. The monomeric Z(HER2â¶342) does not seem to be able to induce receptor phosphorylation and dimerization or sensitize cells to irradiation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tolerância a Radiação
/
Proteínas Recombinantes de Fusão
/
Receptor ErbB-2
Limite:
Humans
Idioma:
En
Revista:
PLoS One
Assunto da revista:
CIENCIA
/
MEDICINA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Suécia