Dissecting single-molecule signal transduction in carbon nanotube circuits with protein engineering.
Nano Lett
; 13(2): 625-31, 2013 Feb 13.
Article
em En
| MEDLINE
| ID: mdl-23323846
ABSTRACT
Single-molecule experimental methods have provided new insights into biomolecular function, dynamic disorder, and transient states that are all invisible to conventional measurements. A novel, nonfluorescent single-molecule technique involves attaching single molecules to single-walled carbon nanotube field-effective transistors (SWNT FETs). These ultrasensitive electronic devices provide long-duration, label-free monitoring of biomolecules and their dynamic motions. However, generalization of the SWNT FET technique first requires design rules that can predict the success and applicability of these devices. Here, we report on the transduction mechanism linking enzymatic processivity to electrical signal generation by a SWNT FET. The interaction between SWNT FETs and the enzyme lysozyme was systematically dissected using eight different lysozyme variants synthesized by protein engineering. The data prove that effective signal generation can be accomplished using a single charged amino acid, when appropriately located, providing a foundation to widely apply SWNT FET sensitivity to other biomolecular systems.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia de Proteínas
/
Transdução de Sinais
/
Muramidase
/
Nanotubos de Carbono
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos