Genome-wide quantitative enhancer activity maps identified by STARR-seq.
Science
; 339(6123): 1074-7, 2013 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-23328393
ABSTRACT
Genomic enhancers are important regulators of gene expression, but their identification is a challenge, and methods depend on indirect measures of activity. We developed a method termed STARR-seq to directly and quantitatively assess enhancer activity for millions of candidates from arbitrary sources of DNA, which enables screens across entire genomes. When applied to the Drosophila genome, STARR-seq identifies thousands of cell type-specific enhancers across a broad continuum of strengths, links differential gene expression to differences in enhancer activity, and creates a genome-wide quantitative enhancer map. This map reveals the highly complex regulation of transcription, with several independent enhancers for both developmental regulators and ubiquitously expressed genes. STARR-seq can be used to identify and quantify enhancer activity in other eukaryotes, including humans.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Mapeamento Cromossômico
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Elementos Facilitadores Genéticos
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Áustria