Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks.
Kidney Int
; 83(6): 1052-64, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23364521
ABSTRACT
A thorough characterization of the transcriptome and proteome of endogenous podocytes has been hampered by low cell yields during isolation. Here we describe a double fluorescent reporter mouse model combined with an optimized bead perfusion protocol and efficient single cell dissociation to yield more than 500,000 podocytes per mouse allowing for global, unbiased downstream applications. Combining mRNA and miRNA transcriptional profiling with quantitative proteomic analyses revealed programs of highly specific gene regulation tightly controlling cytoskeleton, cell differentiation, endosomal transport, and peroxisome function in podocytes. Strikingly, the analyses further predict that these podocyte-specific gene regulatory networks are accompanied by alternative splicing of respective genes. Thus, our 'omics' approach will facilitate the discovery and integration of novel gene, protein, and organelle regulatory networks that deepen our systematic understanding of podocyte biology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
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Perfilação da Expressão Gênica
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Proteômica
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Podócitos
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Redes Reguladoras de Genes
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Proteínas Luminescentes
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Kidney Int
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Alemanha