Genomic inversion caused by gamma irradiation contributes to downregulation of a WBC11 homolog in bloomless sorghum.
Theor Appl Genet
; 126(6): 1513-20, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23463491
ABSTRACT
Epicuticular wax (bloom) plays important roles in protecting the tissues of sorghum (Sorghum bicolor (L.) Moench) plants from abiotic stresses. However, reducing wax content provides resistance to greenbug and sheath blight-a useful trait in agricultural crops. We generated a sorghum bloomless (bm) mutant by gamma irradiation. One bm population segregated for individuals with and without epicuticular wax at a frequency of 7222, suggesting that the bm mutation was under the control of a single recessive nuclear gene. Genes differentially expressed in the wild-type and the bm mutant were identified by RNA-seq technology. Of the 31 downregulated genes, Sb06g023280 was the most differentially expressed and was similar to WBC11, which encodes an ABC transporter responsible for wax secretion in Arabidopsis. An inversion of about 1.4 Mb was present in the region upstream of the Sb06g023280 gene in the bm mutant; it is likely that this inversion changed the promoter sequence of the Sb06g023280 gene. Using genomic PCR, we confirmed that six independent F2 bm mutant-phenotype plants carried the same inversion. Therefore, we concluded that the inversion involving the Sb06g023280 gene inhibited wax secretion in the bloomless sorghum.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cruzamento
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Regulação da Expressão Gênica de Plantas
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Transportadores de Cassetes de Ligação de ATP
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Sorghum
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Inversão de Sequência
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Raios gama
Idioma:
En
Revista:
Theor Appl Genet
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão