Aptamer-directed lanthanide chelate self-assembly for rapid thrombin detection.
Analyst
; 138(17): 5107-12, 2013 Sep 07.
Article
em En
| MEDLINE
| ID: mdl-23807946
ABSTRACT
We report a sensitive assay method for homogeneous thrombin detection. The method is based on lanthanide chelate complementation, where the luminescent complex is split into two separate label moieties, which are intrinsically non-luminescent. A luminescent mixed chelate complex is formed only when the label moieties are brought into close proximity directed by two separate binding events of aptamers to the analyte. This results in high specificity in signal generation while time-resolved fluorescence detection eliminates the short lifetime autofluorescence, which is inherent to many homogeneous assays and limits their applicability. The developed method is also very rapid as the maximum signal is obtained in just five minutes. Lanthanide chelate complementation can be applied for the detection of other proteins when two binders recognizing separate epitopes of the analyte are available.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trombina
/
Técnicas Biossensoriais
/
Quelantes
/
Aptâmeros de Nucleotídeos
/
Európio
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Analyst
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Finlândia