Mode of virus rescue determines the acquisition of VHS mutations in VP22-negative herpes simplex virus 1.
J Virol
; 87(18): 10389-93, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23864617
ABSTRACT
It has been proposed that herpes simplex virus 1 with VP22 deleted requires secondary mutation of VHS for viability. Here we show that a replication-competent Δ22 virus constructed by homologous recombination maintains a wild-type (Wt) VHS gene and has no other gross mutations. By contrast, Δ22 viruses recovered from a bacterial artificial chromosome contain multiple amino acid changes within a conserved region of VHS. Hence, the mode of virus rescue influences the acquisition of secondary mutations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribonucleases
/
Proteínas Virais
/
Herpesvirus Humano 1
/
Mutação
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido