Labeling deoxyadenosine for the preparation of functional conjugated oligonucleotides.
Bioconjug Chem
; 24(8): 1398-407, 2013 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-23883134
ABSTRACT
Herein we present a versatile synthetic method for the 8-thioalkylation of (deoxy)adenosine with a short carbon linker having on the other side a variety of molecules (psoralen, acridine) and functional groups (alkyne). After conventional protections, the modified adenosine can be phosphytylated and inserted into an oligonucleotide without affecting the standard protocols for supported oligonucleotide synthesis. The hybridization properties of a generic oligonucleotide containing the above conjugated moieties toward both DNA and RNA are evaluated both in the case of a perfectly complementary strand and in the case of a single mismatch. This methodology is suitable for the preparation of several types of derivatives andthrough the alkynyl moietyprovides fast access to click-chemistry transformations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligodesoxirribonucleotídeos
/
Coloração e Rotulagem
/
Desoxiadenosinas
Idioma:
En
Revista:
Bioconjug Chem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article