Cloning of cDNA and genomic DNA for human cytochrome P-45011 beta.
FEBS Lett
; 269(2): 345-9, 1990 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-2401360
ABSTRACT
A full-length cDNA clone encoding steroid 11 beta-hydroxylase (P-45011 beta) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-450(11) beta gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-450(11) beta gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroide Hidroxilases
/
DNA de Neoplasias
/
Esteroide 11-beta-Hidroxilase
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Japão