Alteration of urinary macromolecules by adsorption on surfaces, probably an important factor in urolithiasis.
Urolithiasis
; 41(6): 467-74, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-24036974
ABSTRACT
To get information on the consequences of adsorption of urinary macromolecules (UMs) on crystals, Ca phosphate (CaP) precipitation was performed in urine of 15 stone patients and 15 controls. In solutions of dissolved precipitates (DPU), Ca oxalate (CaOx) crystallization and aggregation (AGN) of latex beads were spectrophotometrically studied and compared to results obtained in urine and in UMs isolated by hemofilter dialysis (HD). Tests were repeated with a 20 µg/mL albumin solution (AS). Inhibition of CaOx growth and AGN was significantly reduced in DPU as well as in HD. Furthermore, DPU and HD showed an AGN of latex beads which in patients was more pronounced than in controls. Identical effects observed in DPU and HD can be explained by temporary high concentrations of UMs by adsorption on surfaces favoring self AGN. Temporary adsorption of AS on CaP produced polymers of a limited size, significantly reduced the inhibition of CaOx AGN and accelerated latex AGN. In DPU, AGN of latex beads occurred despite a surface potential of -37 mV, which within short distances produces areas of massive electrostatic repulsion and normally inhibits AGN. Such areas might be bridged by self-aggregated UMs. The retardation of CaOx AGN measured in DPU could be decisive whether during crystalluria crystals are washed out from the kidney by diuresis or are attached to kidney calcifications or pre-existing stones.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Urina
/
Fosfatos de Cálcio
/
Substâncias Macromoleculares
/
Urolitíase
Tipo de estudo:
Observational_studies
Limite:
Humans
Idioma:
En
Revista:
Urolithiasis
Ano de publicação:
2013
Tipo de documento:
Article