Nedd4-2 (NEDD4L) controls intracellular Na(+)-mediated activity of voltage-gated sodium channels in primary cortical neurons.
Biochem J
; 457(1): 27-31, 2014 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-24152020
ABSTRACT
Nedd4-2, a HECT (homologous with E6-associated protein C-terminus)-type ubiquitin protein ligase, has been implicated in regulating several ion channels, including Navs (voltage-gated sodium channels). In Xenopus oocytes Nedd4-2 strongly inhibits the activity of multiple Navs. However, the conditions under which Nedd4-2 mediates native Nav regulation remain uncharacterized. Using Nedd4-2-deficient mice, we demonstrate in the present study that in foetal cortical neurons Nedd4-2 regulates Navs specifically in response to elevated intracellular Na(+), but does not affect steady-state Nav activity. In dorsal root ganglia neurons from the same mice, however, Nedd4-2 does not control Nav activities. The results of the present study provide the first physiological evidence for an essential function of Nedd4-2 in regulating Navs in the central nervous system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sódio
/
Córtex Cerebral
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Ubiquitina-Proteína Ligases
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Complexos Endossomais de Distribuição Requeridos para Transporte
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Canais de Sódio Disparados por Voltagem
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Biochem J
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Austrália