[Synthesis and immunochemical study of the antigenic determinant of the H-2Db molecule of the murine major histocompatibility complex]. / Sintez i immunokhimicheskoe izuchenie antigennoi determinanty H-2Db-molekuly glavnogo kompleksa gistosovmestimosti myshi.
Bioorg Khim
; 12(1): 89-99, 1986 Jan.
Article
em Ru
| MEDLINE
| ID: mdl-2418844
ABSTRACT
Primary structure of murine class I histocompatibility antigens has been analysed to select possible antigenic determinant. Hexapeptide Leu-Gln-Gln-Leu-Ser-Gly, homologous to the region 95-100 of the H-2Db antigen heavy chain, was synthesised by stepwise elongation of peptide chain beginning from the COOH-terminal Gly. Rabbit anti-hexapeptide antibodies were obtained and shown to interact specifically with purified H-2Db antigen as well as with the native antigen on cell surface. These antibodies bind to lymphocytes of H-2b haplotype (C57BL/6 mice) but not H-2d (BALB/c) or H-2k (CBA). These data suggest that the region 95-100 is responsible for serologic differences between the alleles of H-2 antigens, i.e. it may be a xenotypic as well as an allotypic antigenic determinant. The latter was confirmed by study of interaction of the hexapeptide with allogeneic monoclonal antibodies specific to H-2Db antigen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Antígenos H-2
/
Epitopos
Limite:
Animals
Idioma:
Ru
Revista:
Bioorg Khim
Ano de publicação:
1986
Tipo de documento:
Article