Long-term systemic toxicity of shikonin derivatives in Wistar rats.

ABSTRACT

Abstract Context Although the antitumor, immunomodulatory activities, and other effects of shikonin have been studied for decades, its systemic toxicity in vivo remains unclear. Objective: To estimate the long-term systemic toxicity of shikonin derivatives (ShD) in a rat model. Materials and methods: The roots of Arnebia euchroma (Royle) Johnst. (Boraginaceae) were extracted in ethanol, passed through a molecular sieve, and dried. A microemulsion solution in water was subsequently prepared. Adult Wistar rats were treated with ShD by gavage at concentrations of 200, 400, and 800 mg/kg per day for 90 days or 180 days. Hematological and biochemical examinations were performed, and the vital organs were subjected to pathological analyses. Results: We did not observe hematological or non-hematological toxicity of ShD at a dose as high as 800 mg/kg per day for 6 months. Discussion and conclusion: Our findings may offer some beneficial information for the practical application and research of Arnebia euchroma. We demonstrated in an animal model that ShD may be safe for usage.