Selection of bisphenol A - single-chain antibodies from a non-immunized mouse library by ribosome display.
Anal Biochem
; 488: 59-64, 2015 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-24269893
ABSTRACT
Developing reagents with high affinity and specificity are critical to detect the environmental hormones or toxicants. Ribosome display technology has been widely used in functional protein or peptide screening and in directed evolution of protein molecules in vitro. In this study, single-chain variable fragments (scFvs) against bisphenol A (BPA) were selected from a library constructed from splenocytes of non-immunized mice. After five rounds of selection, the selected scFvs bound to BPA with high affinity. Indirect competitive enzyme-linked immunosorbent assay (ELISA) was introduced to screen the antibody affinity and specificity to BPA. The equilibrium dissociation constants (KDS) of one clone was 1.76µM as determined by surface plasmon resonance (SPR). This study indicated that ribosome display can isolate binders to small molecules from a non-immunized naive library without any in vivo steps and can generate recombinant antibodies efficiently and rapidly. In addition, this study provides a methodological framework for detection of small molecules using recombinant antibodies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Ribossomos
/
Compostos Benzidrílicos
/
Poluentes Ambientais
/
Estrogênios não Esteroides
/
Anticorpos de Cadeia Única
País/Região como assunto:
Asia
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China