Protective effect of ginsenoside Rg1 on lidocaine-induced apoptosis.
Mol Med Rep
; 9(2): 395-400, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24270314
ABSTRACT
Lidocaine, as an anesthetic substance, is often used for surface and spinal anesthesia. However, studies have shown that lidocaine may induce transient neurological symptoms and cauda equina syndrome. In the present study the effects of the ginsenoside Rg1 (Rg1) on lidocaineinduced apoptosis were assessed in Jurkat cells using flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The data showed that incubation with Rg1 provides protection against lidocaineinduced apoptosis in cultured Jurkat cells. In order to investigate the effect of Rg1 on the apoptosis pathway, caspase 3 gene expression was determined. The results suggested that the protective effect of Rg1 on lidocaineinduced apoptosis is mediated by altering the level of Bcell lymphoma2 (BCL2) family proteins and downregulating caspase3 expression. In conclusion, the present study demonstrated that incubation with Rg1 provides protection against lidocaineinduced apoptosis in cultured Jurkat cells. In addition, the study demonstrated that Rg1 is a notable antiapoptotic molecule that is capable of blocking the caspasedependent signaling cascade in Jurkat cells, and that the protective effect of Rg1 on lidocaineinduced apoptosis is mediated by altering levels of BCL2 family proteins and downregulating caspase3 expression. The present study provides the basis for understanding and evaluating the effect of Rg1 in the in vivo treatment of lidocaine-induced transient neurological symptoms and cauda equina syndrome by lidocaine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polirradiculopatia
/
Ginsenosídeos
/
Lidocaína
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2014
Tipo de documento:
Article