Human cytomegalovirus suppresses Fas expression and function.
J Gen Virol
; 95(Pt 4): 933-939, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24394698
ABSTRACT
Human cytomegalovirus (HCMV) is known to evade extrinsic pro-apoptotic pathways not only by downregulating cell surface expression of the death receptors TNFR1, TRAIL receptor 1 (TNFRSF10A) and TRAIL receptor 2 (TNFRSF10B), but also by impeding downstream signalling events. Fas (CD95/APO-1/TNFRSF6) also plays a prominent role in apoptotic clearance of virus-infected cells, so its fate in HCMV-infected cells needs to be addressed. Here, we show that cell surface expression of Fas was suppressed in HCMV-infected fibroblasts from 24 h onwards through the late phase of productive infection, and was dependent on de novo virus-encoded gene expression but not virus DNA replication. Significant levels of the fully glycosylated (endoglycosidase-H-resistant) Fas were retained within HCMV-infected cells throughout the infection within intracellular membranous structures. HCMV infection provided cells with a high level of protection against Fas-mediated apoptosis. Downregulation of Fas was observed with HCMV strains AD169, FIX, Merlin and TB40.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptor fas
/
Citomegalovirus
/
Interações Hospedeiro-Patógeno
/
Evasão da Resposta Imune
Limite:
Humans
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Reino Unido