Producing and concentrating lenti-Cre for mouse infections.
Cold Spring Harb Protoc
; 2014(3): 304-6, 2014 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-24591688
ABSTRACT
Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lentivirus
/
Integrases
/
Vetores Genéticos
/
Infecções
Limite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2014
Tipo de documento:
Article