Submicrogram quantities of unstained proteins are visualized on polyvinylidene difluoride membranes by transillumination.
Appl Theor Electrophor
; 1(1): 59-60, 1988.
Article
em En
| MEDLINE
| ID: mdl-2484988
ABSTRACT
Unstained proteins on polyvinylidene difluoride membranes which have been dried and saturated with 20% methanol appear as clear areas when placed on a white light box. Sensitivity is comparable to that obtained with Coomassie Blue R250. This procedure greatly reduces the potential for unintended chemical modification during staining procedures, provides a nondestructive means of determining the major features of a protein pattern on a blot and makes it possible to initiate sequencing within minutes after blotting is complete.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polivinil
/
Proteínas
/
Membranas Artificiais
Idioma:
En
Revista:
Appl Theor Electrophor
Ano de publicação:
1988
Tipo de documento:
Article