The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes NMO class I and II.

Salvi, Francesca; Agniswamy, Johnson; Yuan, Hongling; Vercammen, Ken; Pelicaen, Rudy; Cornelis, Pierre; Spain, Jim C; Weber, Irene T; Gadda, Giovanni

Salvi, Francesca; Agniswamy, Johnson; Yuan, Hongling; Vercammen, Ken; Pelicaen, Rudy; Cornelis, Pierre; Spain, Jim C; Weber, Irene T; Gadda, Giovanni.

Afiliação

Salvi F; From the Departments of Chemistry.
Agniswamy J; Biology.
Yuan H; From the Departments of Chemistry.
Vercammen K; the Department of Bioengineering Sciences, Vrije Universiteit Brussel, Belgium, and the Department of Structural Biology Brussels, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
Pelicaen R; the Department of Bioengineering Sciences, Vrije Universiteit Brussel, Belgium, and the Department of Structural Biology Brussels, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
Cornelis P; the Department of Bioengineering Sciences, Vrije Universiteit Brussel, Belgium, and the Department of Structural Biology Brussels, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
Spain JC; the School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia 30302.
Weber IT; From the Departments of Chemistry, Biology, Center for Biotechnology and Drug Design, iweber@gsu.edu.
Gadda G; From the Departments of Chemistry, Biology, Center for Biotechnology and Drug Design, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia 30302, ggadda@gsu.edu.

J Biol Chem ; 289(34): 23764-75, 2014 Aug 22.

Article em En | MEDLINE | ID: mdl-25002579

Assuntos

Oxigenases de Função Mista/metabolismo; Pseudomonas aeruginosa/enzimologia; Sequência de Aminoácidos; Cristalização; Eletroforese em Gel de Poliacrilamida; Cinética; Oxigenases de Função Mista/química; Dados de Sequência Molecular; Conformação Proteica; Homologia de Sequência de Aminoácidos; Especificidade por Substrato

Palavras-chave

3-Nitropropionate; Enzyme Catalysis; Enzyme Structure; Flavoprotein; Nitronate Monooxygenase; Oxidoreductase; Propionate 3-Nitronate; Pseudomonas Aeruginosa (P. Aeruginosa); TIM Barrel; Toxin

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Oxigenases de Função Mista Idioma: En Revista: J Biol Chem Ano de publicação: 2014 Tipo de documento: Article

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