An improved method for determining the microbial inhibitory activity of serum and its application to the study of patients with leukemia.
Am J Clin Pathol
; 92(2): 192-8, 1989 Aug.
Article
em En
| MEDLINE
| ID: mdl-2502906
ABSTRACT
Numerous investigators have demonstrated that derangements in serum transferrin and iron can contribute to susceptibility to infection, but the complexity and imprecision of assays have impeded both research and development of clinical testing in this area. This article describes an automated assay for measuring the microbial inhibitory activity of transferrin in serum and its use in patients with acute myelogenous leukemia (AML) and in normal controls. The assay measured the ability of heat-inactivated serum to inhibit the growth of an antibiotic-resistant strain of Pseudomonas aeruginosa. The serum dilutions were prepared in a special low iron chemically defined broth. An inhibition index, the reciprocal of the serum dilution producing 50% inhibition of bacterial growth when compared with the growth in broth alone, was determined. The results showed the serum from the patients with leukemia had a significantly lower inhibition index than that of controls (16 +/- 11 vs. 35 +/- 13, P less than 0.01). In addition, they had higher serum iron levels (162 +/- 65 vs. 75 +/- 27, P less than 0.01), lower serum transferrin levels (231 +/- 65 vs. 309 +/- 71, P less than 0.01), and higher percentage saturation of transferrin with iron (59 +/- 21 vs. 20 +/- 8, P less than 0.01) than did controls. Because the assay uses equipment available in many clinical laboratories, it could be developed for routine use as an index of susceptibility to infection in selected patients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Atividade Bactericida do Sangue
/
Transferrina
/
Leucemia Mielomonocítica Aguda
/
Testes de Sensibilidade Microbiana
/
Ferro
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Am J Clin Pathol
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Geórgia