HPLC-ELSD determination of kanamycin B in the presence of kanamycin A in fermentation broth.
Biomed Chromatogr
; 29(3): 396-401, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25042110
ABSTRACT
A novel method for the direct determination of kanamycin B in the presence of kanamycin A in fermentation broth using high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) was developed. An Agilent Technologies C18 column was utilized, evaporation temperature of 40°C and nitrogen pressure of 3.5 bar, the optimized mobile phase was water-acetonitrile (6535, v/v), containing 11.6 mm heptafluorobutyric acid (isocratic elution with flow rate of 0.5 mL/min) with the gain 11. Kanamycin B was eluted at 5.6 min with an asymmetry factor of 1.827. The method showed good linearity over the concentration range of 0.05 to 0.80 mg/mL for the kanamycin B (r(2) = 0.9987). The intra-day and inter-day coefficients of variation obtained from kanamycin B were less than 4.3%. Mean recovery of kanamycin B from spiked fermentation broth was 95%. The developed method was applied to the determination of kanamycin B without any interference from other constituents in the fermentation broth. This method offers simple, rapid and quantitative detection of kanamycin B.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espalhamento de Radiação
/
Canamicina
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2015
Tipo de documento:
Article