A study on the inhibition of VEGF expression in salivary gland adenoid cystic carcinoma cells via iNOS gene RNAi in vitro.
J Oral Pathol Med
; 44(2): 153-8, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25065562
ABSTRACT
In this study, we aimed to explore the effect of inducible nitric oxide synthase (iNOS) on vascular endothelial growth factor (VEGF) expression in salivary gland adenoid cystic carcinoma (SACC). Using RNAi, we transfected chemically synthesised iNOS siRNA into ACC-M cells (a highly metastatic adenoid cystic carcinoma cell line) and detected the change in the gene and protein expression levels of iNOS and VEGF by qRT-PCR and Western blotting. A transwell invasiveness assay was used to examine the changes in invasive ability of ACC-M cells. Cell growth was determined using a CCK-8 assay. Apoptosis and cell-cycle phases were detected by flow cytometry. We found that silencing iNOS down-regulated the expression of VEGF and then inhibited cell growth and invasiveness of SACC cells, while it increased apoptosis. Therefore, we concluded that iNOS can regulate VEGF expression and iNOS may be a therapeutic target.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias das Glândulas Salivares
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Carcinoma Adenoide Cístico
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Fator A de Crescimento do Endotélio Vascular
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Óxido Nítrico Sintase Tipo II
Limite:
Humans
Idioma:
En
Revista:
J Oral Pathol Med
Assunto da revista:
ODONTOLOGIA
/
PATOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China