Effect of mixture of Trichoderma isolates on biochemical parameters in leaf of Macrophomina phaseolina infected brinjal.

ABSTRACT

A mixture of Trichoderma harzianum NBRI-1055 (Fx) and T. harzianum BHU-99 (Th) was evaluated for their efficiency to induce systemic resistance during three way interaction among brinjal-Trichoderma-Macrophomina phaseolina. Total phenol content (TPC), defence related enzymes Phenylalanine ammonia-lyase (PAL), Peroxidase (PO), Polyphenol oxidase (PPO) and PR proteins (PR-2 and PR-3) were recorded. Total phenolic content was recorded 12.82 times and 1.8 times higher in Trichoderma mixture treated-pathogen challenge (Fx-Th-Pth) treatment than in untreated healthy control and untreated pathogen challenged (Pth) plants respectively after 72 hr pathogen inoculation (hapi). Defence related enzymes PAL 4.54 times higher, 48hapi, PO, 3.96 times higher, 72hapi and PPO 8.1 times higher, 72hapi in Fx-Th-Pth treatment than untreated healthy control, and the PR- proteins such as PR-2, 2.15 times and PR-3, 2.16 times higher, 72hapi than untreated healthy control. The results showed that a mixture of Trichoderma (Fx+Th) performed better than single isolate.