Characterization of a novel plasmid-borne thiopeptide gene cluster in Staphylococcus epidermidis strain 115.
J Bacteriol
; 196(24): 4344-50, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25313391
ABSTRACT
Thiopeptides are small (12- to 17-amino-acid), heavily modified peptides of bacterial origin. This antibiotic family, with more than 100 known members, is characterized by the presence of sulfur-containing heterocyclic rings and dehydrated residues within a macrocyclic peptide structure. Thiopeptides, including micrococcin P1, have garnered significant attention in recent years for their potent antimicrobial activity against bacteria, fungi, and even protozoa. Micrococcin P1 is known to target the ribosome; however, like those of other thiopeptides, its biosynthesis and mechanisms of self-immunity are poorly characterized. We have discovered an isolate of Staphylococcus epidermidis harboring the genes for thiopeptide production and self-protection on a 24-kb plasmid. Here we report the characterization of this plasmid, identify the antimicrobial peptide that it encodes, and provide evidence of a target replacement-mediated mechanism of self-immunity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Plasmídeos
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Staphylococcus epidermidis
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Bacteriocinas
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Família Multigênica
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos