Lab-on-a-brain: implantable micro-optical fluidic devices for neural cell analysis in vivo.
Sci Rep
; 4: 6721, 2014 Oct 22.
Article
em En
| MEDLINE
| ID: mdl-25335545
ABSTRACT
The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an interface between neurons and the outer world. The newly developed device provides the functions required for the long-term and high-resolution observation of the fine structures of neurons by two-photon laser scanning microscopy and the microfluidic delivery of chemicals or drugs directly into the brain. A proof-of-concept experiment of single-synapse stimulation by two-photon uncaging of caged glutamate and observation of dendritic spine shrinkage over subsequent days demonstrated a promising use for the present technology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Técnicas Analíticas Microfluídicas
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Japão